Form DOT F 1385 at a glance
What it is: Appendix H to Part 40, the DOT Drug and Alcohol Testing Management Information System (MIS) Data Collection Form, is the form required for use to report calendar year MIS data.
Who signs: Enter the Name of Certifying Official and sign on the Signature line.
A filled-in Form DOT F 1385 example: a motor carrier reports its random tests

How to fill out Form DOT F 1385, box by box
Calendar Year Covered by this Report, Company Name, DBA, Address and E-mail
Name of Certifying Official, Signature, Telephone, Date Certified and Prepared by
Check the DOT agency for which you are reporting MIS data

Check the DOT agency for which you are reporting MIS data: FMCSA - Motor Carrier, FAA - Aviation, PHMSA - Pipeline, FRA - Railroad, USCG - Maritime or FTA - Transit; and complete the information on that same line as appropriate.
On the FMCSA - Motor Carrier line, enter the DOT # and circle YES or NO for Owner-operator and for Exempt.
On the FAA - Aviation line, enter the Certificate # (if applicable) and the Plan / Registration # (if applicable).
On the PHMSA - Pipeline line, check Gas Gathering, Gas Transmission, Gas Distribution, Transport Hazardous Liquids or Transport Carbon Dioxide.
On the FRA - Railroad line, enter the Total Number of observed/documented Part 219 “Rule G” Observations for covered employees.
On the USCG - Maritime line, enter the Vessel ID # (USCG- or State-Issued); if there is more than one vessel, list separately.
II. Covered Employees

Under II. Covered Employees, (A) Enter Total Number Safety-Sensitive Employees In All Employee Categories.
(B) Enter Total Number of Employee Categories.
Under (C), enter each Employee Category and the Total Number of Employees in this Category.
If you have multiple employee categories, complete Sections I and II (A) & (B). Take that filled-in form and make one copy for each employee category and complete Sections II (C), III, and IV for each separate employee category.
III. Drug Testing Data

In III. Drug Testing Data, the Total Number Of Test Results should equal the sum of Columns 2, 3, 9, 10, 11, and 12.
Enter the Verified Negative Results and the Verified Positive Results For One Or More Drugs.
Enter Positive For Marijuana, Positive For Cocaine, Positive For PCP, Positive For Opioids and Positive For Amphetamines.
Under Refusal Results, enter Adulterated, Substituted, “Shy Bladder” With No Medical Explanation and Other Refusals To Submit To Testing.
In III. Drug Testing Data, enter the Cancelled Results.
In III. Drug Testing Data, each Type of Test has its own row: Pre-Employment, Random, Post-Accident, Reasonable Susp./Cause, Return-to-Duty and Follow-Up, then TOTAL.
IV. Alcohol Testing Data

In IV. Alcohol Testing Data, the Total Number Of Screening Test Results should equal the sum of Columns 2, 3, 7, and 8.
On page 2, under Alcohol Testing Data, enter the Screening Tests With Results Below 0.02 and the Screening Tests With Results 0.02 Or Greater.
Enter the Number Of Confirmation Tests Results, the Confirmation Tests With Results 0.02 Through 0.039 and the Confirmation Tests With Results 0.04 Or Greater.
In IV. Alcohol Testing Data, under Refusal Results, enter “Shy Lung” With No Medical Explanation and Other Refusals To Submit To Testing.
In IV. Alcohol Testing Data, enter the Cancelled Results.
In IV. Alcohol Testing Data, each Type of Test has its own row: Pre-Employment, Random, Post-Accident, Reasonable Susp./Cause, Return-to-Duty and Follow-Up, then TOTAL.
More rules for Form DOT F 1385
The instructions for this form are found at https://www.transportation.gov/odapc.
Common questions about Form DOT F 1385
Is there a penalty?
Title 18, USC Section 1001, makes it a criminal offense subject to a maximum fine of $10,000, or imprisonment for not more than 5 years, or both, to knowingly and willfully make or cause to be made any false or fraudulent statements of representations in any matter within the jurisdiction of any agency of the United States (checked November 2, 2026).
Sources
[1] Form DOT F 1385 (Rev. 4/2019), DOT Drug and Alcohol Testing MIS Data Collection Form, U.S. Department of Transportation (the Official source link on this page).



